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Fish samples have been blended with dry ice in blender/homogenizer with pulsed action till contents were uniform and had the consistency of a advantageous powder. 6. Rotary evaporator - Büchi mannequin R-a hundred and ten with ice trap, evaporation temperature 50 °C (Brinkmann Instruments, Inc. Westbury, NY). 10. Volumetric glassware and pipettes - 100.Zero and 50.0 mL volumetric flasks, class A, P/N 28014-one hundred and 28014-50 (Kimble/Kontes, Vineland, NJ); 15 mL graduated centrifuge tubes with glass stoppers 13, P/N 45153-15 (Kimble/Kontes, Vineland, NJ); adjustable volume pipettors with disposable polypropylene tips - 10-a hundred μL Eppendorf (Brinkmann Instruments, Inc., Westbury, NY), 200-a thousand μL (Ulster Scientific, Inc., New Paltz, NY), and 1-5 mL (Wheaton Science Products, Millville, NJ). P/N 81385), with guard column (Alltech C18, 5 μm guard cartridge, 7.5 × 4.6 mm(P/N 96080) (Alltech Associates, Deerfield, IL), and column pre-filter (ColumnSaver, 0.5 μm (P/N MMCS-210), MacMod Analytical Inc., Chadds Ford, PA). 8. Propylsulfonic acid (PRS-SPE) columns - Bond Elut LRC propylsulfonic acid stable phase extraction columns, 500 mg, disposable, P/N 1211-3038, with LRC 10 mL column connection adaptors, P/N 1213-1003 (Varian Inc., Palo Alto, CA). Leucomalachite inexperienced was quantitatively oxidized to the chromic malachite green by reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Samples have been then cleaned-up by solid part extraction with alumina and propylsulfonic acid phases

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