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The Appeal Of A Bet

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작성자 Mitchel Atencio
댓글 0건 조회 12회 작성일 25-02-03 16:34

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Rec-seq also confirmed earlier findings8 that Cre has a pronounced choice in two areas of loxP: half-site positions 5-7 and 10. We observed 5.0-fold enrichment of the canonical base at position 10, in keeping with experiences that Arg259 participates in hydrogen bonding with the C•G base pair at position 10 (refs. Structural and mutagenesis studies23,26,31 suggested that mutation of Arg259 would have an effect on specificity at half-site place 10. Indeed, the Arg259→Ala variant showed a drop in enrichment at position 10 (from 5.0-fold for wild-kind Cre to 1.1-fold for the mutant), with a modest choice for C or T within the left half-site and G or A in the best half-site (Fig. 2b, c). For instance, in helix B, Rec-seq of the Lys43→Ala mutant resulted in a modest drop in specificity relative to wild-kind Cre, while Met44→Ala resulted in greater choice at positions 5 and 10 (Fig. 2d). In helix D, the Lys86→Ala variant showed minimal variations from wild-kind Cre (Supplementary Fig. 7a), whereas the Gln90→Ala variant confirmed general decrease enrichment (Fig. 2e). In the loop between helices J and K, the Arg282→Ala mutant confirmed greater, moderately than decrease, DNA specificity across loxP (Supplementary Fig. 7b). These results display that Cre’s obvious desire at positions 5-7 outcomes from a number of weak or oblique interactions, quite than being strongly determined by residues proximal to those positions

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